Heart rate variability (HRV) is a non-invasive biomarker that reflects autonomic nervous system dynamics, providing valuable insights into physiological adaptation, stress, and recovery in athletes. Among the various HRV metrics, the root mean square of successive differences (RMSSD) has emerged as a robust and practical measure due to its strong association with parasympathetic activity, ease of calculation, and reliability in both short- and ultra-short-term recordings. This review examines the methodological considerations for using HRV to monitor training adaptations and recovery status in athletic populations. We highlight the superiority of routine, near-daily HRV measurements over isolated assessments, emphasizing the utility of weekly averages and the coefficient of variation (CV) to capture both chronic adaptations and acute homeostatic perturbations. Additionally, we discuss the selection of HRV devices, data recording procedures, and strategies to enhance athlete compliance. While RMSSD offers significant advantages for field-based monitoring, we also address its limitations, including its sole focus on parasympathetic activity and susceptibility to external confounders. Future directions include the integration of HRV data with other physiological markers and machine learning algorithms to optimize individualized training and recovery strategies. This review provides sport scientists and practitioners with evidence-based recommendations to enhance the application of HRV in both research and real-world athletic settings.
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